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mers cov pseudovirus  (Sino Biological)


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    Structured Review

    Sino Biological mers cov pseudovirus
    Mers Cov Pseudovirus, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mers+cov+spike/MERS-CoV+Spike+Protein/pm42034896-58-25-31
    Average 95 stars, based on 146 article reviews
    mers cov pseudovirus - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Detection of viral antibodies in camel sera using magnetic particle spectroscopy.
    Article Snippet: Furthermore, 10 μg/ml of the respective individual antigens namely SARS-Cov-2-Spike, SARS-Cov-2 nucleocapsid, and MERS-Cov-Spike (Sino Biological, China) was loaded on the MNP.


    Recombinant:

    Article Title: Ultrasensitive detection of SARS-CoV-2 S protein with aptamers biosensor based on surface-enhanced Raman scattering.
    Article Snippet: A rapid and accurate diagnostic modality is essential to prevent the spread of SARS-CoV-2.. In this study, we proposed a SARS-CoV-2 detection sensor based on surface-enhanced Raman scattering (SERS) to achieve rapid and ultrasensitive detection.. The sensor utilized spike protein deoxyribonucleic acid aptamers with strong affinity as the recognition entity to achieve high specificity.

    Article Title: Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants
    Article Snippet: Pseudotyped SARS-CoV-2 particles (PSV, 10 5 TU μL –1 ; TU, transducing units), coronavirus HKU1, and Influenza A virus (H3N2) were from Fubio Biological Technology Co., Ltd. (China). .. MERS-CoV spike-His recombinant protein (40069-V08H), human coronavirus HKU1 spike-His recombinant protein (40602-V08H), and HIV-1 gp120-His recombinant protein (40402-V08H) were obtained from Sino Biological Inc. (China). .. Chloroauric acid hydrate (HAuCl 4 ·4H 2 O), MgCl 2 , and sodium citrate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

    Article Title: Molecular Crowding Modulates SARS‐CoV‐2 Aptamer Affinity
    Article Snippet: Polyethylene glycol 4000 (PEG 4000) was purchased from Sigma Aldrich. .. His-tagged-RBD of SARS-CoV-2 Spike Protein (40 592-V08B), SARS-CoV spike-His recombinant protein (40 150-V08B2), and MERS-CoV spike-His recombinant protein (40 069-V08H) were purchased from Sino Biological Inc (China). .. All DNA sequences were synthesized by Sangon Biotech (Shanghai, China).

    Article Title: Plasmonic Biosensor Field-Effect Devices for Virus Detection by Carbon Nanomaterials
    Article Snippet: The predominance of viruses is a real threat to human safety as they cause severe infections.. The high prevalence of viral diseases can be attributed to inadequate detection techniques.. Various types of biosensors have been developed and commercialized with the intention of detecting dangerous viruses.

    Article Title: Vaccines against coronavirus and methods of use
    Article Snippet: .. Ninety-six well immunosorbent plates (NUNC) were coated with 1 ug/mL recombinant SARS-CoV-2 S1+S2 ECD protein (Sino Biological 40589-V08B1), S1 protein (Sino Biological 40591-V08H), S2 protein (Sino Biological 40590-V08B), or receptor-binding domain (RBD) protein (Sino Biological 40595-V05H) in DPBS overnight at 4° C. ELISA plates were also coated with 1 ug/mL recombinant SARS-CoV S1 protein (Sino Biological 40150-V08B1) and RBD protein (Sino Biological 40592-V08B) or MERS-CoV Spike (Sino Biological 40069-V08B). .. Plates were washed four times with PBS+0.05% Tween20 (PBS-T) and blocked with 5% skim milk in PBS-T (5% SM) for 90 minutes at 37° C. Sera or BAL from INO-4800 vaccinated macaques were serially diluted in 5% SM, added to the washed ELISA plates, and incubated for 1 hour at 37° C. Following incubation, plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Vaccines against coronavirus and methods of use
    Article Snippet: .. Ninety-six well immunosorbent plates (NUNC) were coated with 1 ug/mL recombinant SARS-CoV-2 S1+S2 ECD protein (Sino Biological 40589-V08B1), S1 protein (Sino Biological 40591-V08H), S2 protein (Sino Biological 40590-V08B), or receptor-binding domain (RBD) protein (Sino Biological 40595-V05H) in DPBS overnight at 4° C. ELISA plates were also coated with 1 ug/mL recombinant SARS-CoV S1 protein (Sino Biological 40150-V08B1) and RBD protein (Sino Biological 40592-V08B) or MERS-CoV Spike (Sino Biological 40069-V08B). .. Plates were washed four times with PBS+0.05% Tween20 (PBS-T) and blocked with 5% skim milk in PBS-T (5% SM) for 90 minutes at 37° C. Sera or BAL from INO-4800 vaccinated macaques were serially diluted in 5% SM, added to the washed ELISA plates, and incubated for 1 hour at 37° C. Following incubation, plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5).

    Plasmid Preparation:

    Article Title: Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography
    Article Snippet: .. Plasmids used for generating pseudovirus stocks were sourced as follows: plasmid encoding an Env -defective, luciferase-expressing HIV-1 genome (pNL4-3.luc.R-E − ) , was obtained through the NIH AIDS Reagent Program; plasmids encoding SARS-CoV-1 and SARS-CoV-2 Spike were from Fang Li (Addgene plasmid #145031 and #145032, respectively) ; WIV1-CoV Spike was from Alejandro Balazs (Addgene plasmid #164439) ; MERS-CoV Spike was from Sino Biological (VG40069-NF); and VSV-G was from Bob Weinberg (Addgene plasmid #8454). .. HEK293T cells (human embryonic kidney), Vero E6 cells and Calu-3 cells were purchased from ATCC.

    Luciferase:

    Article Title: Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography
    Article Snippet: .. Plasmids used for generating pseudovirus stocks were sourced as follows: plasmid encoding an Env -defective, luciferase-expressing HIV-1 genome (pNL4-3.luc.R-E − ) , was obtained through the NIH AIDS Reagent Program; plasmids encoding SARS-CoV-1 and SARS-CoV-2 Spike were from Fang Li (Addgene plasmid #145031 and #145032, respectively) ; WIV1-CoV Spike was from Alejandro Balazs (Addgene plasmid #164439) ; MERS-CoV Spike was from Sino Biological (VG40069-NF); and VSV-G was from Bob Weinberg (Addgene plasmid #8454). .. HEK293T cells (human embryonic kidney), Vero E6 cells and Calu-3 cells were purchased from ATCC.



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